Researchers can define specific regions of interest within the tissue, such as atherosclerotic plaques or hepatocytes, and calculate the percentage of area occupied by lipid droplets. It is widely employed in research concerning metabolic disorders, atherosclerosis, liver pathology, and the assessment of drug-induced steatosis.
Time Course Oil Red O Staining Procedure
The Staining Procedure The standard Oil Red O staining procedure involves two primary solutions: a working stain solution and a rinsing solution. Fresh tissues should be snap-frozen in optimal cutting temperature compound and stored at low temperatures until sectioning.
The differentiation between neutral lipids and other lipid types is essential for accurate interpretation of the results. Unlike paraffin-embedded tissues, frozen sections are required because the organic solvents used in the staining process would dissolve the paraffin wax.
Step-by-Step Time Course Oil Red O Staining Procedure
Principles of Lipid Staining The underlying principle of Oil Red O staining is based on the solubility characteristics of the dye. Counterstaining and Mounting While Oil Red O provides excellent visualization of lipids, a counterstain is often employed to improve the overall morphology of the tissue and provide context for the lipid localization.
More About Oil red o staining
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